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All slides were obtained serially from the section used for Xenium analysis A , Iba-1 immunolabeling of macrophages by DAB chromogen in mouse (left) and rat (right) tumors. Macrophages adjacent areas of necrosis (black arrowhead), in areas of abundant stroma (black arrows) and less stroma (open arrows) are shown. Areas of necrosis (N), capsule (C), denser stroma (*) are indicated. Scale bar indicates 2 mm (4x; top row) and 1 mm, 10x; bottom row). Quantification can be found in Supplemental Figure 12. B , Iba-1 immunolabeling of macrophages by DAB chromogen in stroma-dense (left) or stroma-poor (right) regions of tumors taken from the SRG rat. Scale bar indicates 500 μm (10x). C, <t>CD68/CD163</t> dual IF for macrophages in stroma-dense and stroma-poor regions of SRG rat. DAPI: top row, CD68 (green channel), CD163 (red channel), Merged image: bottom row; CD68+CD163+ cells are yellow. Scale bar indicates 200 μm (30x).
Primary Antibody Incubation, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against cd68 (ed1)  (BMA Biomedicals)
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All slides were obtained serially from the section used for Xenium analysis A , Iba-1 immunolabeling of macrophages by DAB chromogen in mouse (left) and rat (right) tumors. Macrophages adjacent areas of necrosis (black arrowhead), in areas of abundant stroma (black arrows) and less stroma (open arrows) are shown. Areas of necrosis (N), capsule (C), denser stroma (*) are indicated. Scale bar indicates 2 mm (4x; top row) and 1 mm, 10x; bottom row). Quantification can be found in Supplemental Figure 12. B , Iba-1 immunolabeling of macrophages by DAB chromogen in stroma-dense (left) or stroma-poor (right) regions of tumors taken from the SRG rat. Scale bar indicates 500 μm (10x). C, <t>CD68/CD163</t> dual IF for macrophages in stroma-dense and stroma-poor regions of SRG rat. DAPI: top row, CD68 (green channel), CD163 (red channel), Merged image: bottom row; CD68+CD163+ cells are yellow. Scale bar indicates 200 μm (30x).
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mouse igg1  (Novus Biologicals)
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List of antibodies used in the experiments.
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All slides were obtained serially from the section used for Xenium analysis A , Iba-1 immunolabeling of macrophages by DAB chromogen in mouse (left) and rat (right) tumors. Macrophages adjacent areas of necrosis (black arrowhead), in areas of abundant stroma (black arrows) and less stroma (open arrows) are shown. Areas of necrosis (N), capsule (C), denser stroma (*) are indicated. Scale bar indicates 2 mm (4x; top row) and 1 mm, 10x; bottom row). Quantification can be found in Supplemental Figure 12. B , Iba-1 immunolabeling of macrophages by DAB chromogen in stroma-dense (left) or stroma-poor (right) regions of tumors taken from the SRG rat. Scale bar indicates 500 μm (10x). C, CD68/CD163 dual IF for macrophages in stroma-dense and stroma-poor regions of SRG rat. DAPI: top row, CD68 (green channel), CD163 (red channel), Merged image: bottom row; CD68+CD163+ cells are yellow. Scale bar indicates 200 μm (30x).

Journal: bioRxiv

Article Title: The SRG RAT® supports human cell xenotransplantation through enhanced tumor microenvironment interactions

doi: 10.1101/2025.03.27.645250

Figure Lengend Snippet: All slides were obtained serially from the section used for Xenium analysis A , Iba-1 immunolabeling of macrophages by DAB chromogen in mouse (left) and rat (right) tumors. Macrophages adjacent areas of necrosis (black arrowhead), in areas of abundant stroma (black arrows) and less stroma (open arrows) are shown. Areas of necrosis (N), capsule (C), denser stroma (*) are indicated. Scale bar indicates 2 mm (4x; top row) and 1 mm, 10x; bottom row). Quantification can be found in Supplemental Figure 12. B , Iba-1 immunolabeling of macrophages by DAB chromogen in stroma-dense (left) or stroma-poor (right) regions of tumors taken from the SRG rat. Scale bar indicates 500 μm (10x). C, CD68/CD163 dual IF for macrophages in stroma-dense and stroma-poor regions of SRG rat. DAPI: top row, CD68 (green channel), CD163 (red channel), Merged image: bottom row; CD68+CD163+ cells are yellow. Scale bar indicates 200 μm (30x).

Article Snippet: Primary antibody incubation (CD68: 1:300, mouse mAb [ED1], Novus Biologicals #NB600-985; CD163: 1:500, rabbit mAb [EPR19518], Abcam #ab182422) was performed at room temperature for 30 minutes.

Techniques: Immunolabeling

List of antibodies used in the experiments.

Journal: Frontiers in Immunology

Article Title: Role of RGS17 in cisplatin-induced cochlear inflammation and ototoxicity via caspase-3 activation

doi: 10.3389/fimmu.2025.1470625

Figure Lengend Snippet: List of antibodies used in the experiments.

Article Snippet: CD68 , Mouse IgG1 , 1:100 , Novus (NB600-985).

Techniques: